Detection of Mycoplasma infection in cows with pneumonic lesions by conventional and PCR method and measuring the antigenic proffle of the isolated mycoplasma

Three different laboratory diagnostic tests were used for rapid and accurate diagnosis and typing of different Mycoplasma strains causing respiratory diseases in cows. A total of hundred lung samples from cows, with pneumqnic lesions were collected from El-Basateen abattoir, and examined for the presence of Mycoplasma, by using isolation, polymerase chain reaction [PCR] and measuring the protein profile of the different isolates using [SDS-PAGE]. The results indicted that Mycoplasma bovis showed the highest percentage among other mycoplasma isolates [M. arginin and M. bovirhinis and untyped strains]. The result reported that the PCR was more rapid, sensitive and specific for accurate detection of Mycoplasma infection in comparison with culture method that was more specific, most difficult and laborious of time and effort. The presence of specific protein bands at 112, 96, 65, 50, 41, 30, and 17 KDa in M.bovis isolates was characteristic. Slight differences in the expression levels of some proteins could be detected

application of a recent technique for diagnosis of mycoplasma gallisepticum infection from migratory quail

Twenty two Mycoplasma isolates were recoverd from 120 Coturuix coturuix quails at Ismailia governorate. Identification and serotyping of these isolates revealed 9 M. gallisepticum [MG], 8M. gallinarum, and 5M. pullorum. Experimental infection was designed where 10 chicks [one week old] were inoculated intranasally with 0.2 ml 10[7] CFU/ml M. gallisepticum isolated from quails, 10 chicks were inoculated with M. gallisepticum S6 strain and 10 chicks were considered as control negative. Post mortem [PM] lesions were milder in chicks inoculated with M. gallisepticum isolated from quail compared to those inoculated with M. gallisepticum S6 strain and presented by congested lungs and liver, turbidity, and thickening of air sacs. Histopathological examination showed that the lungs of chicks infected with M. gallisepticum isolated from quail showed oedema with mononuclear leucocytic inflammatory cells infiltration in the interlobular connective tissue stroma. The grade of histopathological examination of tracheas of chicks infected with M.G. isolated from quail showed local lymphoid cell aggregation of leucocytic inflammatory cells in the lamina propria underneath the epithelial mucosa was mild. Mycoplasma gallisepticum could be reisolated and identified from challenged. Serologic antibody response was less in sera of chicks infected with M. gallisepticum isolated from quails. Polymerase chain reaction confirmed that the Mycoplasma isolates were M. gallisepticum

Detection of different bacteria causing decreased hatchability in turkey ecgs

A total of 360 eggs collected from turkey farms, from low fertility and low hatchability, were examined for both Mycoplasma and egg borne bacterial diseases. Fresh fertile eggs revealed the recovery of Mycoplasma meleagridis [50%] and Salmonella typhimurium [20%]. From non-fertile eggs, Mycoplasma iowae [50%], Mycoplasma gallopavonis [25%] were isolated. No bacteria were recovered from the non-fertile eggs. From dead embryos several Mycoplasma species were isolated viz., Mycoplasma iowae [25%], Mycoplasma meleagridis [25%] and Mycoplasma gallopuvonis [25%]. Mixed infection with coliforms were isolated from all the internal contents of the eggs and dead embryos